![]() ![]() And usually, data obtained with a high voltage above 550-600V are considered doubtful. So, the less light you have, the higher the high voltage is. The high voltages used by the CD detector during measurements are directly linked to light levels. This is because oxygen absorbs light in the far UV and the deeper you go in the UV the more it absorbs (see figure1). But it is also true that nitrogen use should be limited to far-UV needs and not used with near UV, visible, or NIR regions. The main reason given is the need to remove oxygen and to reduce its light absorption in the far-UV range. Why waste money on nitrogen for Circular Dichroism?įor decades, some Circular Dichroism (CD) instrument manufacturers have made nitrogen (N 2) flushing of optics an absolute requirement for CD measurements, but is it always really necessary? The answer is no. ![]()
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